Tissue Culture Microscopes are often used in biological studies when examining living tissues which are placed on petri dishes and flask. Tissue culture samples are frequently observed in petri dishes and must be viewed from the base of the dish. By the way petri dish is a shallow, transparent dish with a flat lid that is commonly used for culture microorganism. This demands the microscope to be modified; it must be an inverted tissue culture microscope that has objectives pointing directly up. This is the reverse version of a typical compound light microscope that has objectives pointing downward which are used to view the microscope slide.
A tissue culture microscope needs objective lens and condensers that can work from a long distance to examine the specimen under the petri culture dish. A standard compound biological light microscope can see thin specimens on a microscope slide with generally 0.17mm cover glass on the slide. These objectives are commonly marked 0.17mm, but on the inverted tissue culture microscope can possibly have objectives which are labeled LWD which stands for long working distance. In that way, you will not be mistaken on what objectives to use on both microscopes. The distance of this objective means the space starting from the objective's glass going to the top part of the sample when it is focus. A standard tissue culture microscope is also suitable for phase contrast microscopy wherein you can view unstained living organism, living cells and tissues. The feature of phase contrast enables wide viewing contrast for living tissue or cell samples that will usually appear transparent when not stained.
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